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ABSTRACT
We aimed to investigate the effect of Type l diabetes mellitus duration and its metabolic control on cellular immunity parameters and the relation of PPD with these tests. In our study, 20 type l diabetic patients and 18 healthy people which made up the control group were included. T lymphocyte count by T-Rosette method and CD4, CD8, CD16 lymphocyte counts by monoclonal antibody technique were applied. HbA1c levels were determined for metabolic control degrees. A PPD skin test which indicates delayed cellular immunity was conducted on the patients and control group, then the tests were evaluated after 72 hours. Lymphocyte, T lymphocyte, CD4 ratios and counts; CD8 count and CD4/CD8 lymphocyte ratio in the type l diabetics group were significantly lower than in the control group (p=0.001, p=0.001, p=0.001, p=0.01, p=0.001). PPD evaluations (mm) in the diabetic group were found statistically less significant than in the control group. Lymphocyte, T lymphocyte, CD4 ratios and counts; CD8 count and CD4/CD8 lymphocyte ratio were significantly lower in poorly controlled diabetic cases (HbA1c>7%) than in the well controlled group (HbA1c<7%) (p=004, p=0.01, p=0.004, p=0.002, p=0.04). PPD evaluations (mm) were significantly lower in poorly controlled cases than in well controlled cases (p=0.04). There were negative correlations between HbA1c and CD4/CD8 lymphocyte ratio, lymphocyte, T lymphocyte, CD4 ratios and counts. The lymphocyte and T lymphocyte ratios and CD4/CD8 lymphocyte ratio were significantly lower in patients with a long duration of diabetes (>5 years), than in those with a short duration of diabetes (>=5 years) (p=0.01, p=O.03, p=0.01). In conclusion, we decided that the cellular immunity decreases in type l diabetes. The lymphocyte, T lymphocyte, CD4 ratios and CD4/CD8 lymphocyte ratio decrease in type l diabetes. Poor control and long duration of diabetes cause impairment of cellular immunity. PPD. which is a delayed cellular immunity parameter, has low sensitivity in type l diabetes mellitus.
We aimed to investigate the effect of Type l diabetes mellitus duration and its metabolic control on cellular immunity parameters and the relation of PPD with these tests. In our study, 20 type l diabetic patients and 18 healthy people which made up the control group were included. T lymphocyte count by T-Rosette method and CD4, CD8, CD16 lymphocyte counts by monoclonal antibody technique were applied. HbA1c levels were determined for metabolic control degrees. A PPD skin test which indicates delayed cellular immunity was conducted on the patients and control group, then the tests were evaluated after 72 hours. Lymphocyte, T lymphocyte, CD4 ratios and counts; CD8 count and CD4/CD8 lymphocyte ratio in the type l diabetics group were significantly lower than in the control group (p=0.001, p=0.001, p=0.001, p=0.01, p=0.001). PPD evaluations (mm) in the diabetic group were found statistically less significant than in the control group. Lymphocyte, T lymphocyte, CD4 ratios and counts; CD8 count and CD4/CD8 lymphocyte ratio were significantly lower in poorly controlled diabetic cases (HbA1c>7%) than in the well controlled group (HbA1c<7%) (p=004, p=0.01, p=0.004, p=0.002, p=0.04). PPD evaluations (mm) were significantly lower in poorly controlled cases than in well controlled cases (p=0.04). There were negative correlations between HbA1c and CD4/CD8 lymphocyte ratio, lymphocyte, T lymphocyte, CD4 ratios and counts. The lymphocyte and T lymphocyte ratios and CD4/CD8 lymphocyte ratio were significantly lower in patients with a long duration of diabetes (>5 years), than in those with a short duration of diabetes (>=5 years) (p=0.01, p=O.03, p=0.01). In conclusion, we decided that the cellular immunity decreases in type l diabetes. The lymphocyte, T lymphocyte, CD4 ratios and CD4/CD8 lymphocyte ratio decrease in type l diabetes. Poor control and long duration of diabetes cause impairment of cellular immunity. PPD. which is a delayed cellular immunity parameter, has low sensitivity in type l diabetes mellitus.